有机锡、有机铅和有机汞对人类及动植物有毒性，因此食品、纺织、玩具、环境等领域都需要针对这些化合物进行检测，以保障人们的健康安全。

 

目前，针对这些化合物已开发出多种检测及定量分析方法，其中大部分基于气相色谱(GC)，并结合各种灵敏性且具高选择性的检测方法，例如：原子吸收光谱(AAS)，原子发射光谱(AES)，脉冲火焰光度检测(PFPD)和质谱(MS)等。基于气相色谱(GC)的检测方法，需对分析物先进行衍生化以增加其挥发性， 同时衍生化还可防止分析过程中（尤其是检测阶段）的干扰。专一且可定量的衍生化方法可避免分析物的损失或降解，对分析结果至关重要。 最常用的方法是使用格氏试剂或四乙基硼酸钠进行氢化和烷基化。

 

四乙基硼酸钠是理想的衍生化试剂

 

 

Cat. No.: 11-0575

Product Name: Sodium tetraethylborate, min. 98%

CAS: 15523-24-7

性质：白色结晶粉末，对空气和水敏感，并且会自燃。可作为烷基化试剂，与各种有机金属反应活性高。

 

因其对含有机金属的水溶性样品进行衍生化时，将在原位进行反应，从而避免了其他操作步骤。乙基化产物的收率取决于与金属相连烷基的取代度及类型、pH值和反应时间。例如：有机锡化合物检测最合适的pH值为4-5。

 

另外，由于样品中含有的其他金属也会参与反应消耗试剂，因此必须使用过量的NaBEt4[1]。

 

除GC外，四乙基硼酸钠衍生化还可用于多种光谱检测方法，并应用于多种环境样品中有毒有机金属物质分析，示例如下：

 

Devos, Christophe, Luc Moens, and Pat Sandra. "Determination of the acaricide fenbutatin oxide in water samples by automated headspace‐SPME‐GC/MS." Journal of separation science 28, no. 7 (2005): 665-668.

 

Orellana-Velado, Néstor Guillermo, Rosario Pereiro, and Alfredo Sanz-Medel. "Solid phase microextraction gas chromatography-glow discharge-optical emission detection for tin and lead speciation." Journal of Analytical Atomic Spectrometry 16, no. 4 (2001): 376-381.

 

Perna, Lorenzo, Angela LaCroix-Fralish, and Stefan Stürup. "Determination of inorganic mercury and methylmercury in zooplankton and fish samples by speciated isotopic dilution GC-ICP-MS after alkaline digestion." Journal of Analytical Atomic Spectrometry 20, no. 3 (2005): 236-238.

 

Larsson, Tom, Erik Björn, and Wolfgang Frech. "Species specific isotope dilution with on line derivatisation for determination of gaseous mercury species." Journal of analytical atomic spectrometry 20, no. 11 (2005): 1232-1239.

 

Bowles, Karl C., Simon C. Apte, and Leigh T. Hales. "Determination of butyltin species in natural waters using aqueous phase ethylation and off-line room temperature trapping." Analytica chimica acta 477, no. 1 (2003): 103-111.

 

Clark, Steven, and Peter J. Craig. "The use of sodium tetraethylborate for the derivatization and analysis of selenium containing compounds." Microchimica Acta 109, no. 1-4 (1992): 141-144.

 

Mota, J. Pablo Valles, M. Rosario Fernández de la Campa, and Alfredo Sanz-Medel. "Merging zones flow injection for the determination of ultratraces of bismuth by volatile species generation atomic absorption spectrometry using sodium tetraethylborate (III)." Journal of Analytical Atomic Spectrometry 13, no. 5 (1998): 431-435.

 

Ndungu, Kuria, and Lennart Mathiasson. "Microporous membrane liquid–liquid extraction technique combined with gas chromatography mass spectrometry for the determination of organotin compounds." Analytica chimica acta 404, no. 2 (2000): 319-328.

 

参考文献：

Morabito, Roberto, Paolo Massanisso, and Philippe Quevauviller. "Derivatization methods for the determination of organotin compounds in environmental samples." TrAC Trends in Analytical Chemistry 19, no. 2-3 (2000): 113-119.

 

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